The ecotoxicity of a mixture of the ionic liquid ethylammonium nitrate (EAN) and aluminum nitrate salt (Al(NO3)3) as well as the corresponding to pure components was studied in this work. This mixture has singular interest as electrolyte in electrochemical applications and data about the effects of mixture and components on the environment can hardly be found in literature. Changes on the bioluminescence of the Aliivibrio fischeri (Beijerinck) Urbanczyk bacteria through Microtox® test and on microbial activity, measured by microcalorimetry, of two soils with different organic matter content, when exposed to solutions of different concentrations of these compounds, were analyzed.