The conservation of wild and local varieties of plants contributes to sustainable agricultural production, healthy and diversified diets, healthy ecosystems and sociocultural stability. However, FAO estimates that over the last century about 75 percent of the genetic diversity of agricultural crops has been lost. An increasing number of local varieties of fruit trees are in danger of loss through habitat destruction or socio-economic pressures towards the use of commercial and more uniform varieties. Tissue culture represents a method for conservation and propagation of these genetic resources, promoting their reintroduction in the agricultural sector. The aim of this study was to micropropagate three varieties of cherry growing at local farms in Galicia, Northwestern Spain. Plant material of the varieties named “de Viño”, “Negra de San Cristobo” and “Negra de Fene” was provided by a local association of fruit growers (Agfa do Eume). Shoots developed in branches of four-year old trees were used for the initiation of in vitro cultures. For proliferation, cherry germplasm was cultured in semisolid medium gelled with agar and in liquid medium by temporary immersion, using RITA® bioreactors. For rooting induction, indole-3-butyric acid was applied at 2 mg L-1 for one month or at 25 mg L-1for 24 h. For rooting expression, shoots were cultured in glass jars in semisolid medium or in plantform™ bioreactors with rockwool cubes soaked in liquid medium. One min aeration with CO2 enriched air was provided 16 times per day to the plantform™ bioreactors. The three varieties of cherry were successfully proliferated in semisolid and liquid medium. Multiplication coefficient and shoot length were affected by genotype in both systems. Rooting ability was also genotype dependent. Shoots rooted in plantform™ bioreactors showed more roots and performed better during the acclimation process than those rooted in semisolid medium.