In this study we present the micropropagation of an ancient almond tree, probably 300- years-old, together with juvenile material proceeding from its seeds. Three types of material were used: 1) shoots flushed on the tree at the beginning of spring, 2) shoots forced to flush in a phytotron from branch segments collected in late winter, and 3) seeds collected in autumn and stored at a cool place for six months before being germinated in vitro. Murashige and Skoog medium supplemented with 0.5 mg L-1N6-benzyladenine and 0.05 mg L-1indole-3-butyric acid was used for culture establishment and stabilization. Different combinations of plant growth regulators were evaluated for shoot proliferation and elongation. Roots formed spontaneously in the multiplication medium. Rooted shoots from cultures obtained from the mother tree and from lines that had been obtained from small seedlings were successfully acclimatized in the phytotron and the greenhouse.