Salix viminalis is a species with a high capacity for micropropagation and acclimation, and could therefore be used to develop emergent techniques in the field of plant propagation. The aims of this study were to propagate willow in liquid medium, both by temporary and continuous systems, to explore the application of photoautotrophic conditions and to investigate the acclimation of willow plantlets in different types of soils that could be used as alternatives for commercial peat. The results indicate that liquid medium under photomixotrophic and under photoautotrophic conditions can be satisfactorily used to culture willow axillary shoots. Regarding acclimation, although there were some differences in aerial growth, the willow plants were successfully acclimated in all soils.